The estimation of heparin in blood.
نویسنده
چکیده
The estimation of heparin in blood by a quantitative chemical method has been attempted by Jaques (1939), Jaques, Monkhouse,. and Stewart (1949), and Monkhouse and Jaques (1950), but several difficulties were encountered. The method of Charles and Scott (1933) needs a very large quantity of blood and is not practical for use in man or small laboratory animals. A method has therefore been devised which gives satisfactory results with 2 ml. of blood: it is based on a preliminary extraction of heparin from plasma with alkali, then removal of proteins by ammonium sulphate and heat, and finally precipitation of heparin with a basic dye (Holmgren and Wilander, 1937; MacIntosh, 1941), which is then estimated colorimetrically.
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 6 1 شماره
صفحات -
تاریخ انتشار 1953